HM400
Service Description
BGI has extensive experience in the field of targeted metabolomics spanning accurate quantification of target metabolites, verification of hypothesis by untargeted metabolomics or other omics, and metabolic model study for specific metabolites1.
The HM400 panel uses LC-MS/MS technology for high-throughput targeted metabolomics detection. It covers around 400 metabolites including 20 metabolite classes addressing key pathophysiological processes relevant in gut microbiota research and cancer research areas.
Project Workflow
Mass Spectrometry Service Specification
HM400 high-throughput targeted metabolomics services are performed using Waters ACQUITY UPLC and SCIEX QTRAP 6500+.
Sample Preparation and Services
- Custom-developed UPLC-MRM/MS methods combine isotope internal standard technology
- Increase metabolome depth of coverage using ACQUITY UPLC BEH C18
- Using LC-MS (SCIEX QTRAP 6500+) to increase the scope of detection
Quality Standard
- Summary includes all methods and data analysis
- Reports provided in Excel or PDF format; Raw files available upon request
Turn Around Time
Typical 5-7 weeks after sample receipt for raw data delivery
Sample Requirements
We accept metabolite samples in a variety of formats. Contact your BGI account representative for details for your samples.
|
SAMPLE TYPE |
RECOMMENDED SAMPLE AMOUNT |
MINIMUM SAMPLE AMOUNT |
|
Serum, plasma, urine |
≥ 100 µL |
≥ 50 µL |
|
Animal and clinical tissues, feces and intestinal contents, microorganism |
≥ 200 mg |
≥ 25 mg |
|
Cell |
1×10^7 |
1×10^5 |
|
Culture medium, fermentation medium |
≥ 1 mL |
≥ 100 µL |
Data Analysis
- Data analysis performed with metaX1 and BGI library
- Data quality control
- Standard curves
- Quantification results
