MassARRAY Genotyping
Description
Sequenom MassARRAY iPLEX™ platform is based on Matrix Assisted Laser Desorption Ionization Time-of-flight Mass Spectrometr y (MALDI-TOF MS) technology applied in BGI for genotyping. With many advantages such as flexibility of experimental design and accurate genotyping results, this technology is also cost-effective, when testing hundreds or thousands of samples for tens of SNP loci. We are able to conduct HPV, HBV and cancer-related genes detection based on genotyping through MassARRAY platform to meet the demands of high-throughput SNP analysis in the post genomic era.
Advantages
- High sensitivity: able to distinguish differences in quality of 1 Dalton molecules in suppor t of DNA pooling technology, the accuracy >99.7%.
- Full-mechanized operations, automation of data analysis, accurate verification, quality control and eliminate human error.
- Multi-reaction system: simultaneous analysis of each response to hole up to 36 SNPs.
- Automation of data analysis: real-time detection, SNP loci direct reading, automatic classification.
Applications
- SNP genotyping
- Individualized drug therapy for cancer–KRAS EGFR , etc.
- Genotype test services for human papilloma virus (HPV)
- Drug-resistant gene detection of the hepatitis B virus (HBV)
Sample Requirements
- DNA amount: ≥ 1 μg DNA for 1~100 SNP sites
≥2 μg DNA for more than 100 SNP sites.
- Concentration: ≥ 50 ng /μL.
- Purity: OD260/280=1.8~2.0.
Completion Indicator
For the samples which pass the DN A - O D test, success rate of SNP-calling is no less than 50%; For the samples which pass the internal quality control, success rate of SNP-calling is no less than 90%.
Turnaround Time
For 100 samples (each sample with 25 SNP sites), the turnaround time is about 30 business days. Actual turnaround time is related to the amount of sample and SNP sites.
