MeDIP/DIP Sequencing

Description

Methylated DNA immunoprecipitation (MeDIP) is a large-scale (chromosome- or genome-wide) techn ology that is used for analyzing methylated DNA sequences. Methylated DNA fragments are isolated using an antibody raised against 5-methylcytosine (5mC), the purifie d fraction of methylated DNA then undergoes next generation sequencing.  MeDIP-Seq is sensitive to highly methylated, high CpG densities.

Advantages

• Whole genome cover of all methylated regions.
• Enrichment-based method, DMRs analysis with low cost.

Services Specification

Sequencing Strategy: 50PE.

Recommended Data Amount: 4G clean data.

 Sample Requirements

• DNA amount: ≥ 5 μg DNA.
• Concentration: ≥ 50 ng/μL.
• Purity: OD260/280=1.8~2.0, without RNA contamination

 Turnaround Time

The standard d tur n around time for the w workflow, only including standard bioinformatics analysis, is ~45 business days for one sample.