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Metagenomic Sequencing

Description

Metagenomics studies of genetic material that is directly recovered from environmental samples, have benefitted greatly from advanced NGS technology as a method for the exploration of microbial biodiversity. Our metagenomics survey service applies whole genome shotgun sequencing of DNA isolated from environmental samples, with the advantages of high throughput and high coverage. It can provide information not only on species composition and abundance, but also on functional genes, gene differences between samples, metabolic pathways and gene resource mining for bioactive products.

Our Metagenomic Sequencing service applies whole genome shotgun sequencing of DNA that is directly recovered and isolated from complex environmental or human sample types, including the gastrointestinal tract, soil and water.

Metagenomic Sequencing not only provides information on species composition and abundance, but also on functional genes, gene differences between samples, metabolic pathways and provides a rich data resource for mining for bioactive products such as novel enzymes and other biocatalysts.

Our sequencing scale and proprietary technology ensures highly competitive pricing while our extensive experience across a vast range of metagenomics projects ensures our experts are able to provide custom bioinformatics solutions that can help elucidate the relationship between host-associated microbial communities and host phenotype.

 

Key Service Details

  • DNBSEQ Metagenomics Survey Sequencing services are executed with the DNBSEQ sequencing system.
  • Reports and output data files are delivered in industry standard file formats: BAM, .xls, .png and FASTQ data.
  • 150bp Paired-End sequencing.

 

Sequencing Quality Standard

  • PE100 or PE150
  • Guaranteed 85% of bases with quality score of Q20
  • Sequencing data depend on sample types.

 

Sample Requirements

Regular Samples:

-Intact genomic DNA ≥ 1.5μg.

-Concentration ≥ 20 ng/μl.

-Volume 15 μl.

Low Input Samples:

-Intact genomic DNA ≥ 200 ng.

-Concentration ≥ 2.5 ng/μl.

-Volume 15 μl.

 

Turnaround Time

  • Typical 40 working days from sample QC acceptance to cleaned raw data availability.
  • Expedited services are available.