Metaproteomics

Project Workflow

DDA analysis can be carried out if there are no suitable library construction samples:

• Protein extraction
• Protein digestion
• Individual samples
• LC-MS/MS (DDA)

DIA analysis can be carried out if there are suitable library construction samples

Mass Spectrometry Service Specification

Metaproteomics services are performed using nano-flow liquid chromatography and cutting-edge mass spectrometer (Q Exactive HF/HF-X and Orbitrap Fusion Lumos).

Sample Preparation and Services

• The QC analysis of enzymatic hydrolysis is strictly controlled by LC-MS
• DDA-Spectral Library generated using HPLC technology and sample fractionation

Quality Standard

• Summary includes all methods and data analysis
• Reports provided in Excel or PDF format, RAW files available upon request

Turn Around Time

Typical 4-5 weeks from sample QC acceptance to data report delivery

Data Analysis

• Data analysis and validation performed with Mascot
• Protein Analysis
• Species analysis
• Diversity analysis
• Function Analysis
• Correlation Analysis