Nanoproteomics
Mass Spectrometry Service Specification
Nanoproteomics services are performed using nano-flow liquid chromatography and cutting-edge Orbitrap Fusion Lumos mass spectrometer.
Sample Preparation and Services
- The QC of enzymatic hydrolysis is strictly controlled by LC-MS
- DDA-Spectral Library generated using HPLC technology and sample fractionation
- 2h/fraction; 2h for DDA analysis
Quality Standard
- Summary includes all methods and data analysis
- Reports provided in Excel or PDF format, RAW files available upon request
Turn Around Time
Typical 5-6 weeks from sample QC acceptance to data report delivery
Sample Requirements
| SAMPLE TYPE | AMOUNT | NOTE | |
| Library construction samples | Mammalian cells from similar or adjacent parts | 5×105 | More than 50 µg protein can be extracted |
|
Tissue from similar or adjacent parts |
10 mg | ||
|
Nanoscale samples |
Mammalian cells | > 100 | Collected cells or tissues (not LCM) with 200 µL low adsorption PCR tube and stored in 2-5 µL PBS solution |
| Egg cell or early embryo cell | > 1, and 3-5 is better | ||
| Tissue | 1 mg | ||
| LCM (Laser capture microdissection) | Thickness of 10 µm, > 10 mm2 | ||
* By default, it include two parts; but nanoscale samples analysis can also be carried out if there are no suitable library construction samples
Unit 405, Number 54, Erfan tower, 17 Alley, Riazi Bakhshayesh street, Saadat abad, Tehran
+98 (21) 22387349
info[at]intellomix.com
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