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Post Translational Modification (PTM) Proteomics

Service Description
We have developed innovative solutions for optimizing the processes of PTM identification of purified protein, modified protein profiling, and protein PTM quantification in complex biological matrices and other complex mixtures.


Post-translational modification (PTM) of proteins refers to the chemical changes proteins may undergo after translation. Common PTMs include phosphorylation, glycosylation, acetylation, and ubiquitination. Identifying and understanding PTMs is key to studying cell biology and the treatment and prevention of disease.
BGI has extensive experience in the field of PTM Proteomics and has developed reliable workflows using market-leading technologies and an advanced bioinformatics infrastructure.

 

Project Workflow

Phosphorylation:

  • Protein extraction
  • Protein digestion
  • Isobaric labeling and enrichment/Modified peptides enrichment
  • HPLC fractionation
  • LC-MS/MS (DDA for Isobaric labeling and enrichment)
  • LC-MS/MS (DIA for Modified peptides enrichment)
  • Data analysis

 Glycosylation/Acetylation/ Ubiquitylation:

  • Protein extraction
  • Protein digestion
  • Modified peptides enrichment
  • HPLC fractionation
  • LC-MS/MS (DDA)
  • Data analysis

 

Mass Spectrometry Service Specification

PTM Proteomics services are performed using nano-flow liquid chromatography and cutting-edge mass spectrometer (Q Exactive HF/HF-X and Orbitrap Fusion Lumos).

 

Sample Preparation and Services

  • Digestion performed using sequencing-grade trypsin
  • Off-line sample fractionation using HPLC technology
  • Each fraction analyzed using nano-flow LC-MS/MS
  • TiO₂/ZrO₂ enrichment for phosphoproteomics; CST antibody enrichment for acetylated/ ubiquitylated proteins; HILIC column enrichment for glycosylated proteins

 

Quality Standard

  • Summary includes all methods and data analysis
  • Reports provided in Excel or PDF format, RAW files available upon request

 

Turn Around Time

  • Phosphorylation Turn Around Time from sample QC acceptance to data report delivery 3-5 weeks
  • Glycosylation Turn Around Time from sample QC acceptance to data report delivery 4-5 weeks
  • Acetylation Turn Around Time from sample QC acceptance to data report delivery 3-5 weeks

 

Data Analysis

  • Data analysis and validation performed with Mascot
  • Protein PTM identification
  • Protein PTM quantification
  • Modified protein function annotation
  • Differential modified protein function enrichment
  • Kinase prediction analysis
  • Motif distribution of PTM sites