Home
Services
Research Services
Clinical and Diagnostics Services
Direct To Customer Services
Animal, Plant and Microorganisms
FAQ
Blog
Contact
App
Sign In
English 
English  English
Farsi  Farsi / فارسی

Rapid DDA Proteomics

Project Workflow

  • PROTEIN EXTRACTION
  • PROTEIN DIGESTION
  • HPLC FRACTIONATION
  • LC-MS/MS (DDA)
  • DATA ANALYSIS

 

Mass Spectrometry Service Specification

Rapid DDA Proteomics services are performed using nano-flow liquid chromatography and cutting-edge mass spectrometer (Q Exactive HF/HF-X and Triple TOF 5600).

 

Sample Preparation and Services

  • The QC of enzymatic hydrolysis is strictly controlled by LC-MS
  • 2h for DDA analysis

 

Quality Standard

  • Summary includes all methods and data analysis
  • Reports provided in Excel or PDF format, RAW files available upon request

 

Turn Around Time
Typical 3-4 weeks from sample QC acceptance to data report delivery

 

Sample Requirements

SAMPLE TYPE

AMOUNT AND CONCENTRATION

MINIMUM SAMPLE VOLUME

Protein sample in liquid solution

Recommended 400 µg with fractionation: 2 µg/µL

200 µL

Protein sample in liquid solution

Minimum Required 100 µg with fractionation: 1 µg/µL

100 µL

 

 Data Analysis

  • Data analysis and validation performed with Mascot
  • Protein identification and quantification list
  • Differential proteins data statistics and volcano plot
  • Principal component analysis (PCA) and cluster analysis
  • Protein GO/COG/KOG/Pathway annotation
  • GO/COG/KOG enrichment analysis of differential proteins
  • Protein-protein interaction analysis
  • Protein subcellular localization analysis
  • Time series analysis
  • Repeatability analysis