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Targeted Proteomics

Project Workflow

  • Sample preparation
  • Targeted LC-MS/MS analysis
  • Peptide quantitation

 

Mass Spectrometry Service Specification

Targeted Proteomics services are performed using nano-flow liquid chromatography and cutting-edge mass spectrometer (Q Exactive HF/HF-X and QTRAP 6500).

 

Sample Preparation and Services

Customized LC method and MRM/PRM-acquisition MS method

 

Quality Standard

  • Summary includes all methods and data analysis
  • Reports provided in Excel or PDF format, RAW files available upon request

 

Turn Around Time

Typical 8 weeks from sample QC acceptance to data report delivery

 

METHOD DEVELOPMENT

  • Summary of target proteins
  • Data quality
  • Method demonstration

 

SAMPLE QUANTIFICATION

  • Quantitative information
  • Data normalizatio
  • Quantification of target peptide

 

Sample Requirements 

SAMPLE TYPE      AMOUNT
 RECOMMENDED MINIMUM
Animal Animal internal organs (heart, liver, spleen, lung, kidney, skin, muscle, brain, etc.)   ≥ 10 mg   ≥ 1 mg
Mollusks (Taxoplasma, Schistosomiasis, Drosophila, Acarid, Plutella xylostella, Laodelphax, Cestode, Cicada, Hematodinium, etc.)   ≥ 10 mg   ≥ 2 mg
 Cell  Suspended cells, adherent cells   ≥ 1×107   ≥ 1×106
Cell culture supernatant   ≥ 10 ml
 Exosome Exosome isolated by customer   ≥ 50 µg, ≥ 0.5 µg/µL 
Fluid   Plasma, serum (remove highly abundant protein)   ≥ 200 µL   ≥ 200 µL
Plasma, serum (with highly abundant protein)   ≥ 10 µL   ≥ 3 µL
Amniotic fluid, cerebrospinal fluid, semen, etc.
(remove highly abundant protein)		   ≥ 1 mL   ≥ 500 µL
Amniotic fluid, cerebrospinal fluid, semen, etc.
(with highly abundant protein)		   ≥ 200 µL   ≥ 100 µL
Saliva, milk   ≥ 200 µL   ≥ 100 µL
Urine   ≥ 30 mL    ≥ 15 mL
Tear   ≥ 15 µL   ≥ 10 µL
Plant  Twigs of plants (leaf buds, tender leaves), algea    ≥ 300 mg     ≥ 200 mg
Old leaves, roots, stems, bark of plants
 		    ≥ 1 g     ≥ 500 mg
Plant buds, pollen
 		    ≥ 100 mg     ≥ 50 mg
Plant seeds (rice/wheat seeds, etc.), fruits (apples, peaches, pears)     ≥ 1 g     ≥ 500 mg
Microorganism Prokaryotic bacteria (E. coli, Staphylococcus aureus, etc.), fungi (yeast, etc.)    Thallus ≥ 50 mg, cells ≥ 5×106
Protein solution Complex protein solution, Protein powder  ≥ 100 µg,
 ≥ 0.5 µg /µL		  ≥ 50 µg,
 ≥ 0.5 µg /µL
Others Feces   ≥ 300 mg   ≥ 100 mg