Untargeted Metabolomics
Untargeted Metabolomics
Service Description
BGI has extensive experience in the field of untargeted metabolomics with developed reliable workflows using market leading technologies and an advanced bioinformatics infrastructure.
By applying state-of-the-art LC-MS/MS systems and techniques, BGI offers validated workflows of untargeted metabolomics to support biomedical and biotechnology applications¹. Our Untargeted Metabolomics services are designed to obtain a metabolite profile and screen for differentially-expressed molecules in the sample.
Project Workflow
Mass Spectrometry Service Specification
Untargeted Metabolomics services are performed using Waters ACQUITY UPLC and cutting-edge mass spectrometer (Q Exactive HF/HF-X and Q-TOF 5600).
Sample Preparation and Services
- Increase metabolome depth of coverage using both ACQUITY UPLC BEH C18 and Amide column
- (+) and (-) ESI detection by a Q Exactive HF/HF-X mass spectrometer
Quality Standard
- Summary includes all methods and data analysis
- Reports provided in Excel or PDF format, RAW files available upon request
Turn Around Time
Typical 5-7 weeks after sample receipt for raw data delivery
Sample Requirements
We accept metabolite samples in a variety of formats. Contact your BGI account representative for details for your samples.
|
SAMPLE TYPE |
RECOMMENDED SAMPLE AMOUNT |
MINIMUM SAMPLE AMOUNT |
|
Serum, plasma |
≥ 300 µL |
≥ 100 µL |
|
Urine |
≥ 300 µL |
≥ 100 µL |
|
Animal and clinical tissues |
≥ 200 mg |
≥ 25 mg |
|
Feces and intestinal contents |
≥ 200 mg |
≥ 25 mg |
|
Cell |
1×10^7 |
5×10^6 |
|
Microorganism |
≥ 200 mg |
≥ 25 mg |
|
Culture medium, fermentation medium |
≥ 1 mL |
≥ 100 µL |
|
Milk |
≥ 1 mL |
≥ 100 µL |
|
Other body fluids (amniotic fluid, saliva, hemolymph, cerebrospinal fluid, etc.) |
≥ 300 µL |
≥ 100 µL |
Data Analysis
- Data analysis performed with metaX1 and BGI library
- T-test, PCA, PLS-DA, OPLS-DA for differential metabolite identification
- Cluster analysis
- Metabolite pathway annotation
- ROC analysis
- Metabolite correlation analysis
