Whole Genome Bisulfite Sequencing
Description
Whole Genome Bisulfite Sequencing (WGBS) provides a scalable solution for cell characterization and gene expression profiling of hundreds to millions of cells. Methylation of DNA at the fifth position in cytosine (5-mC) is a stable epigenetic modification and plays an important role in many biological processes, including gene silencing, suppression of transposable elements, genomic imprinting and X chromosome inactivation. Detection and quantification of methylation are critical to understand gene expression and other processes subjected to epigenetic regulation.
Whole genome bisulfite sequencing (WGBS) is used to detect methylated cytosines by treating the DNA with sodium bisulfite before sequencing. WGBS has become the gold standard for studying genome-wide methylation at single base resolution.
Sequencing Service Specification
Our Whole Genome Bisulfite Sequencing Services are executed with DNBSEQ technology.
Key Service Details
Besides clean data, we offer a range of standard and customized bioinformatics options for your whole genome bisulfite sequencing project.
Reports and output data flies are delivered in industry standard file formats: FASTQ, BAM, cout, .xls, .png
Sample preparation and services
- Library preparation, including bisulfite treatment
- 100bp paired-end sequencing
- Clean data, standard and customized data analysis
- Available data storage and bioinformatics applications
Sequencing Quality Standard
- Guaranteed ≥85% of clean bases with quality score of Q20
- Guaranteed ≥99% bisulfite conversion rate
- Standard sequencing coverage ≥30X is recommended
Sample Requirements
Regular Samples: Intact genomic DNA ≥ 1μg, Concentration ≥ 50ng/μl
Low Input Samples: Intact genomic DNA ≥ 100ng, Concentration ≥ 7ng/μl
Turnaround Time
- Typical 40 working days from sample QC acceptance to filtered raw data availability
- Expedited services are available
