16S/18S/ITS Sequencing

Description

Over 99% of natural microorganisms cannot be isolated and cultured clonally, while traditional isolation and culture-dependent methods challenge the study of microorganisms in their natural environment. Metagenomic studies of genetic material that is directly recovered from environmental samples, have benefitted greatly from advanced NGS technology as a method for the exploration of microbial biodiversity.

16S and 18S rDNA are hypervariable regions in the 16S or 18S rRNA genes in bacteria and fungus, while ITS (Internal Transcribed Spacer) is the spacer DNA between the small-subunit and large-subunit rRNA genes in bacteria, fungi and archaea.

Sequence comparison of 16S/18S/ITS regions is widely used in taxonomy and molecular phylogenetics because these regions have a high degree of variation even between closely related species, while also offering easy amplification by PCR, even from low quantities of DNA.

We have extensive experience supporting partners across the world with their 16S, 18S rDNA and ITS research needs, while our economies of sequencing scale and proprietary technology ensures extremely efficient pricing.

Key Service Details

• Reports and output data files are delivered in industry standard file formats: BAM, .xls, .png and FASTQ data.
• Our 16S/18S/ITS Amplicon Sequencing services are executed with the Illumina HiSeq 2500 or MiSeq sequencing system.
• PE250 sequencing by either MiSeq or HiSeq 2500, and PE300 by MiSeq.

Sequencing Quality Standard

• Up to ≥ 80% of bases with a ≥ Q30 quality score, depending on the chosen sequencing strategy.
• Recommended sequencing coverage is dependent on the complexity of the sample.

Sample Requirements

Genomic DNA

-Concentration: ≥ 50ng.

-Volume: ≥ 6ng/μl.

PCR Fragment

-Concentration: ≥ 1μg.

-Volume: ≥ 10ng/μl.

Turnaround Time

• Typical 45 working days from sample QC acceptance to cleaned raw data availability.
• Expedited services are available.