Long Non-Coding RNA Sequencing (lncRNA-Seq)

Description

The long chain non-coding RNAs (hereinafter referred to long non-coding RNAs, lncRNAs) are RNAs of which length is greater than 200nt and not involved in protein coding. They are widely distributed in vivo. lncRNAs are involved in various biological process, including epigenetic, the transcriptional and post transcriptional regulation. They have an important role in life activity. Because most lncRNAs do not have polyA tail structure, conventional RNA-Seq method by using Oligo dT magnetic beads for polyA enrichment cannot obtain lncRNA information comprehensively. The long chain non-coding RNA sequencing (lncRNA-Seq) is a research method which uses a specific approach such as the Ribo-Off Series Kit, to firstly reduce rRNA in the sample, then constructs the library based on the enriched RNA, finally perform the bioinformatics analysis on the high-throughput sequencing data output. Therefore, lncRNA information in specific biological processes (such as development, disease, etc.) can be fully and accurately obtained.

Long non-coding RNAs (lncRNAs) are thought to encompass nearly 30,000 different transcripts in humans, hence lncRNA transcripts account for the major part of the non-coding transcriptome. The lncRNA discovery is still at an early stage and only a small proportion of lncRNAs have so far been investigated. Although we can start to classify different types of lncRNA functions, we are still far from being able to predict the function of new lncRNAs.

We offers expression profiling as one way to uncover the function of lncRNA. Identifying lncRNAs that are differentially expressed during development or in particular situations can shed light on their potential functions. Alternatively, looking for lncRNAs and protein-coding genes whose expression is correlated, can indicate co-regulation or related functions.

Compared to the research of protein-coding sequence and small RNA sequence, the research of lncRNA is at its new stage of development, of which function, and regulation mechanism remains to be further clarified. The current research results show the variety of molecular biology functions of lncRNA, such as regulation of transcription patterns, regulation of the activity of the protein, changing the RNA splicing pattern and so on. These regulation areas are still new in the field. lncRNA has been the hotspots in the field of functional genomics after mRNA and microRNA. It has huge potential in market. The conventional technique for lncRNA research such as in situ hybridization, over-expression technology, siRNA-mediated gene silencing technology and real-time quantitative PCR, focusing on the analysis, the lack of efficiency on the part of the target sequence; of lncRNA microarray technology appear, flux high, but has obvious limitations: Microarray is designed for a specific species, generally only provide people applicable version in mice and other species use restricted; Microarray by using probes can detect known lncRNA, but due to currently limited known lncRNA available, limited probe coverage of the chip design, cannot conduct a comprehensive analysis of the samples in the lncRNA; The chip cannot get the novel lncRNA sequence information, and therefore cannot predict novel lncRNA; The new generation of high-throughput sequencing technology combined with bioinformatics prediction tools, so that people will be able to quickly and efficiently discovery has important regulatory functions lncRNA, Fast Track is open for research work.

Advantages

• High mRNA expression correlation and library construction repeatability (Pearson value ≥ 0.90)
• Access to almost all lncRNAs information at one single sequencing run
• Analyze both known lncRNAs and predicted novel lncRNAs
• Applied to all eukaryotic species (lncRNA-seq for species other than human and mouse belongs to customized project).

Key Service Details

Reports and output data files are delivered in industry standard file formats: BAM, .xls, .png and FASTQ data.

Sequencing Standards

• Guaranteed ≥80% of bases with quality score of ≥Q30.
• ≥8 Gb sequencing data per sample is recommended.

Sample Requirements

Human / Mouse / Rat / Non-whole blood:

Total RNA ≥ 200ng.

Concentration ≥ 20ng/μl.

General Quality Requirement: RIN ≥ 7.0.

Turnaround Time

• Typical 30 working days from sample QC acceptance to filtered data availability.
• Expedited services are available, contact us for more detail.

FAQ

Q1: What kind of library prep kits we use for DNBSEQ (DNBSEQ TECHNOLOGY) long-non coding RNA?

A1: We use probes to remove the human/mouse/rat rRNA, followed with stranded library preparation procedure to get the final lncRNA library.

Q2: Why we need have the stranded information for LncRNA sequencing?

A2: The stranded RNA-seq retains strand information of a read, we can resolve read ambiguity in overlapping genes transcribed from anti-sense strands, which provides a more accurate quantification of gene expression levels compared with traditional non-stranded RNA-seq. Since lots of lncRNA overlap with coding genes, stranded specific protocol is necessary for lncRNA studies.

Q3: Do the lncRNA sequencing data only contain lncRNA sequences?

A3: No, the lncRNA library prep protocol, both mRNAs and lncRNA in the sequencing data.

Q4: What species can we sequencing for DNBSEQ LncRNA?

A4: For standard lncRNA sequencing, only human mouse and rat are compatible. For other species, pls refer to the Customized product details.